In vitro cytotoxicity of nanoAg-SiO2 polyurethane materials

doi:10.3969/j.issn.2095-4344.2013.03.003

Abstract

Abstract:

BACKGROUND: We synthesized nanoAg-SiO2 polyurethane during preliminary research for improvement of antibacterial property of polyurethane materials.
OBJECTIVE: To compare the in vitro cytotoxicity of seven kinds of polyurethane materials with different content of nanoAg-SiO2.
METHODS: We melted nanoAg-SiO2 into polyurethane to prepare polyurethane materials containing 0%, 0.5%, 1.0%, 1.5%, 2.0%, 2.5% and 5.0% nanoAg-SiO2, respectively. Then we prepared the leaching liquor of high density polyethylene (negative control), 0.1% phenol fluid (positive control) and these nanoAg-SiO2 polyurethane materials to culture L929 cells for 24, 48 and 72 hours. Cells cultured in RPMI 1640 medium containing 10% fetal bovine serum served as reagent control group, and blank control group was also set. Then MTT colorimetric method was used to quantitatively detect cell relative growth rate and conduct toxicity reaction grading. Cell morphology was observed under microscope.
RESULTS AND CONCLUSION: Cell relative growth rate was greater than 80% and the toxicity reaction ranked level 1 in all polyurethane groups, reagent control group and negative control group. In the above-mentioned groups, cells adhered to the cavity wall with normal appearance, plump soma and cytoplasm under microscope; in vitro cytotoxicity of these polyurethane materials reduced and their biocompatibility improved when the nanoAg-SiO2 content decreased. Cell relative growth rate became lower (P < 0.05) as culture-time prolonged and reduced to 8.7% after 72 hours in the positive control group. Cells cultured in positive control group drifted in the leaching liquor with their trophy and round shape. Seven kinds of polyurethane materials with different contents of nanoAg-SiO2 have good in vitro cellular compatibility and comply with the requirements of in vitro experiments for medical materials with their toxicity reaction ranking level 1.

Key words: biomaterials, nano-biological materials, nanoAg-SiO2, polyurethane, in vitro, L929 cells, relative growth rate, cytotoxicity, antibacterial properties, MTT colorimetric method, provincial grants-supported paper, biomaterial photographs-containing paper

CLC Number:

R318

Yang Liu-cheng, Xu Shuai, Li Shi-si, Wu Kai, Wang Jian-jun. In vitro cytotoxicity of nanoAg-SiO2 polyurethane materials[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(3): 392-399.

Figures/Tables 3

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